Journal: Human Genomics

Article Title: Emerging strategies and applications of pharmacogenomics

doi: 10.1186/1479-7364-1-6-444

Figure Lengend Snippet: Exploiting whole-genome approaches . Candidate gene-based studies have been the focus of human genetic association studies for the past several years. As the knowledge of genetic variation has emerged and the genotyping technologies to investigate such variation have matured, our ability to carry out whole-genome association studies appears poised to address fundamental issues, such as the association with complex diseases and drug responses.

Article Snippet: In an attempt to validate the haplotype tagging approach to identify genetic associations, as well as to evaluate the Perlegen Sciences array-based chip-based genotyping platform, 7,283 SNPs spanning 17.1 megabases (Mb) of DNA were genotyped to identify associations with HDL levels [ ].

Techniques:

Journal: Genetics

Article Title: Oxidative Stress Survival in a Clinical Saccharomyces cerevisiae Isolate Is Influenced by a Major Quantitative Trait Nucleotide

doi: 10.1534/genetics.111.128256

Figure Lengend Snippet: Peroxide survival candidate QTL as identified by BSA and SSA. Shown is a combination of all four S98 BSA and 4x2k SSA genotyping analyses. (A) S98 one-mismatch SFPs, (B) S98 two-mismatch SFPs, (C) 4x2k SFPs with gaps, (D) 4x2k ≥3 consecutive SFPs. The black horizontal line at the bottom is the S. cerevisiae genome with its chromosomes indicated by roman numerals between the beginning (left) and end (right) of each chromosome (short vertical bars). Chromosome lengths are drawn to scale. Candidate QTL are marked by short horizontal bars. Red bars represent loci that were found in the most conservative analysis (B) and at least one other analysis. These regions are highlighted by red vertical lines and their approximate position on the chromosome is indicated at the bottom. Black arrows point to candidate QTL that have been tested with diagnostic RFLPs (Table 2).

Article Snippet: Our approach to dissecting the genetic basis of peroxide survival was to (i) identify at least one QTL derived from the high-survival parent YJK1272, (ii) confirm genetic linkage, and (iii) characterize a gene or even nucleotide that affects the phenotype by applying an economic mapping approach that combined genotyping data obtained from the more costly Affymetrix S98 array with the inexpensive Combimatrix 4x2k array that not only contains four arrays per chip but can be stripped and reprobed twice.

Techniques: Diagnostic Assay